rabbit polyclonal antibody vegf sc-152  (Santa Cruz Biotechnology)

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Ocimum sanctum Linn. Extract Improves Cognitive Deficits in Olfactory Bulbectomized Mice via the Enhancement of Central Cholinergic Systems and VEGF Expression

doi: 10.1155/2021/6627648

Figure Lengend Snippet: Primer sets for target genes in real-time PCR.

Article Snippet: The membranes were blocked in a 5% nonfat milk for 1 h at RT and then probed with VEGF rabbit polyclonal antibody (A-20: sc-152, 1 : 1000 dilution, Santa Cruz Biotechnology) and β -actin rabbit polyclonal antibody (PA1-183, 1 : 2000 dilution, Thermo scientific) at 4°C overnight.

Techniques: Sequencing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Ocimum sanctum Linn. Extract Improves Cognitive Deficits in Olfactory Bulbectomized Mice via the Enhancement of Central Cholinergic Systems and VEGF Expression

doi: 10.1155/2021/6627648

Figure Lengend Snippet: Effects of OS and DNP treatment on the expression levels of VEGF gene (a) and VEGFR2 gene (b) in the hippocampi of OBX mice using real-time PCR. β -Actin mRNA was used to normalize the quantities of VEGF and VEGFR2 genes. Each data column represents the mean ± SEM ( n = 6-7). ∗ p < 0.05 versus sham group and # p < 0.05 versus vehicle-treated OBX group.

Article Snippet: The membranes were blocked in a 5% nonfat milk for 1 h at RT and then probed with VEGF rabbit polyclonal antibody (A-20: sc-152, 1 : 1000 dilution, Santa Cruz Biotechnology) and β -actin rabbit polyclonal antibody (PA1-183, 1 : 2000 dilution, Thermo scientific) at 4°C overnight.

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Ocimum sanctum Linn. Extract Improves Cognitive Deficits in Olfactory Bulbectomized Mice via the Enhancement of Central Cholinergic Systems and VEGF Expression

doi: 10.1155/2021/6627648

Figure Lengend Snippet: Effects of OS and DNP treatment on VEGF expression level in the hippocampi of OBX mice. (a) Representative photographs of western blotting data analysis. (b) Quantitative comparisons of the expression level of VEGF. Each data column represents the mean ± SEM ( n = 5). Values are mean ± SEM. ∗∗ p < 0.01 versus sham group; ∗ p < 0.05 versus vehicle-treated OBX group.

Article Snippet: The membranes were blocked in a 5% nonfat milk for 1 h at RT and then probed with VEGF rabbit polyclonal antibody (A-20: sc-152, 1 : 1000 dilution, Santa Cruz Biotechnology) and β -actin rabbit polyclonal antibody (PA1-183, 1 : 2000 dilution, Thermo scientific) at 4°C overnight.

Techniques: Expressing, Western Blot

Journal: Heliyon

Article Title: High glucose conditioned neonatal astrocytes results in impaired mitogenic activity in cerebral microvessel endothelial cells in co-culture

doi: 10.1016/j.heliyon.2019.e01795

Figure Lengend Snippet: Astrocytes cultured in high glucose media had significantly decreased VEGF mRNA levels compared to normal glucose media (Difference between the means -88.42 ± 2.91%, n = 12, p < 0.0001).

Article Snippet: After blocking with 5% Non-fat dried milk-TBST (Tris Buffer Saline-0.1% Tween 20), membranes were incubated overnight at 4 °C with primary antibody, rabbit polyclonal IgG against VEGF (sc-152, Santa Cruz Biotechnology, Dallas, TX, USA) with 1:200 dilution in TBST-1% BSA.

Techniques: Cell Culture

Journal: Heliyon

Article Title: High glucose conditioned neonatal astrocytes results in impaired mitogenic activity in cerebral microvessel endothelial cells in co-culture

doi: 10.1016/j.heliyon.2019.e01795

Figure Lengend Snippet: Astrocytes cultured in high glucose media had significantly decreased VEGF protein level compared to normal glucose media (n = 4, p < 0.01).

Article Snippet: After blocking with 5% Non-fat dried milk-TBST (Tris Buffer Saline-0.1% Tween 20), membranes were incubated overnight at 4 °C with primary antibody, rabbit polyclonal IgG against VEGF (sc-152, Santa Cruz Biotechnology, Dallas, TX, USA) with 1:200 dilution in TBST-1% BSA.

Techniques: Cell Culture

Journal: Heliyon

Article Title: High glucose conditioned neonatal astrocytes results in impaired mitogenic activity in cerebral microvessel endothelial cells in co-culture

doi: 10.1016/j.heliyon.2019.e01795

Figure Lengend Snippet: Astrocytes cultured in high glucose media secreted significantly decreased levels of VEGF into media compared to normal glucose media (n = 6, *p < 0.001).

Article Snippet: After blocking with 5% Non-fat dried milk-TBST (Tris Buffer Saline-0.1% Tween 20), membranes were incubated overnight at 4 °C with primary antibody, rabbit polyclonal IgG against VEGF (sc-152, Santa Cruz Biotechnology, Dallas, TX, USA) with 1:200 dilution in TBST-1% BSA.

Techniques: Cell Culture